Otimização do cultivo de Ulva ohnoi em sistemas de bioflocos: Influência da salinidade
DOI:
https://doi.org/10.20950/1678-2305/bip.2026.52.e997Palavras-chave:
Tolerância à salinidade, Macroalga, Produção de biomassa, Compostos antioxidantes, Aquicultura integradaResumo
Este estudo avaliou a tolerância à salinidade da macroalga Ulva ohnoi cultivada em um sistema de bioflocos ao longo de um período de 14 dias. Foram testados cinco tratamentos de salinidade (10, 15, 20, 25 e 30‰), utilizando tanques de 40 L. As algas foram aclimatadas às salinidades-alvo antes da fase de cultivo e estocadas na densidade de 2 g∙L-1. Foram avaliados os parâmetros de qualidade da água (amônia, nitrito, nitrato, ortofosfato, alcalinidade e pH), o ganho de biomassa, a taxa de crescimento diário, compostos fenólicos, clorofila e carotenoides. A qualidade da água permaneceu de acordo com as faixas aceitáveis em todos os tratamentos, embora a alcalinidade tenha apresentado diferenças significativas. Mortalidade total de U. ohnoi foi observada em 10‰, e crescimento reduzido foi registrado em 15‰. Em contraste, as salinidades de 20, 25 e 30‰ proporcionaram maiores ganhos de biomassa e taxas de crescimento diário, sem diferenças significativas nas concentrações de compostos antioxidantes entre esses tratamentos. O crescimento foi significativamente maior nas salinidades de 20–30‰, com ganhos de biomassa entre 318–400 g e taxas de crescimento diário de 5,4–6,4% dia-1, enquanto em 15‰ o ganho de biomassa atingiu apenas ~111 g e a taxa de crescimento ~1,2% dia-1. Esses resultados destacam o potencial de U. ohnoi para integração em sistemas de bioflocos de baixa salinidade e em estratégias de aquicultura multitrófica, contribuindo para práticas aquícolas sustentáveis por meio da reciclagem de nutrientes e da produção de biomassa de valor agregado.
Referências
Alamrousi, A., Casais, E., García-Cardesín, É., Masaló, I., Pintado, J., & Cremades, J. (2022). Influence of pH, N, P, N:P ratio, and dissolved inorganic carbon on Ulva ohnoi growth and biomass quality: Potential implications in IMTA-RAS. Aquaculture Journal, 2(4), 285-301. https://doi.org/10.3390/aquacj2040017
Ale, M. T., Mikkelsen, J. D., & Meyer, A. S. (2011). Differential growth response of Ulva lactuca to ammonium and nitrate assimilation. Journal of Applied Phycology, 23, 345-351. https://doi.org/10.1007/s10811-010-9546-2
Angell, A. R., Mata, L., Nys, R., & Paul, N. A. (2015). Indirect and direct effects of salinity on the quantity and quality of total amino acids in Ulva ohnoi (Chlorophyta). Journal of Phycology, 51(3), 536-545. https://doi.org/10.1111/jpy.12300
APHA, AWWA & WEF (2012). Standard Methods for the Examination of Water and Wastewater (22nd ed.). American Public Health Association.
Ben-Ari, T., Neori, A., Ben-Ezra, D., Shauli, L., Odintsov, V., & Shpigel, M. (2014). Management of Ulva lactuca as a biofilter of mariculture effluents in IMTA system. Aquaculture, 434, 493-498. https://doi.org/10.1016/j.aquaculture.2014.08.034
Bews, E., Booher, L., Polizzi, T., Long, C., Kim, J. H., & Edwards, M. S. (2021). Effects of salinity and nutrients on metabolism and growth of Ulva lactuca: Implications for bioremediation of coastal watersheds. Marine Pollution Bulletin, 166, 112199. https://doi.org/10.1016/j.marpolbul.2021.112199
Carvalho, A., Braga, Í., Chaar, F., Cardozo, A. P., Monserrat, J. M., Ramírez, J. R. B., Wasielesky Jr., W., & Poersch, L. H. (2024). Production of the macroalgae Ulva lactuca integrated with the shrimp Penaeus vannamei in a biofloc system: effect of total suspended solids and nutrient concentrations. Phycology, 4(1), 37-52. https://doi.org/10.3390/phycology4010002
Carvalho, A., Costa, L. C. O., Holanda, M., Gonçalves, M., Santos, J., Costa, C. S. B., Turan, G., & Poersch, L. H. (2023). Growth of the macroalgae Ulva lactuca cultivated at different depths in a biofloc integrated system with shrimp and fish. Phycology, 3(2), 280-293. https://doi.org/10.3390/phycology3020018
Chopin, T., Buschmann, A. H., Halling, C., Troell, M., Kautsky, N., Neori, A., Kraemer, G. P., Zertuche-González, J. A., Yarish, C., & Neefus, C. (2001). Integrating seaweeds into marine aquaculture systems: A key toward sustainability. Journal of Phycology, 37(6), 975-986. https://doi.org/10.1046/j.1529-8817.2001.01137.x
Cikos, A. M., Jerković, I., Molnar, M., Šubarić, D., & Jokić, S. (2021). New trends for macroalgal natural products applications. Natural Product Research, 35(7), 1180-1191. https://doi.org/10.1080/14786419.2019.1644629
Cotas, J., Leandro, A., Monteiro, P., Pacheco, D., Figueirinha, A., Gonçalves, A. M., Silva, G. J., & Pereira, L. (2020). Seaweed phenolics: from extraction to applications. Marine Drugs, 18(8), 384. https://doi.org/10.3390/md18080384
Eismann, A. I., Reis, R. P., Silva, A. F., & Cavalcanti, D. N. (2020). Ulva spp. carotenoids: Responses to environmental conditions. Algal Research, 48, 101916. https://doi.org/10.1016/j.algal.2020.101916
Fort, A., Monteiro, J. P., Simon, C., Domingues, M. R., & Sulpice, R. (2024). Short term decreases in salinity, combined with the right choice of species, can allow for a more nutritious sea lettuce lipid profile. Food Chemistry, 437(Pt 1), 137865. https://doi.org/10.1016/j.foodchem.2023.137865
Imchen, T. (2012). Recruitment potential of a green alga Ulva flexuosa Wulfen dark preserved zoospore and its development. PLoS One, 7(2), e32651. https://doi.org/10.1371/journal.pone.0032651
Kakinuma, M., Coury, D. A., Sato, Y., Yokoyama, A., Obika, H., & Miyachi, S. (2006). Physiological and biochemical responses to thermal and salinity stresses in a sterile mutant of Ulva pertusa (Ulvales, Chlorophyta). Marine Biology, 149, 97-106. https://doi.org/10.1007/s00227-005-0215-y
Kakinuma, M., Kuno, Y., & Amano, H. (2004). Salinity stress responses of a sterile mutant of Ulva pertusa (Ulvales, Chlorophyta). Fisheries Science, 70(6), 1177-1179. https://doi.org/10.1111/j.1444-2906.2004.00921.x
Khhoi, L. V., & Fotedar, R. (2011). Integration of western king prawn (Penaeus latisulcatus Kishinouye, 1896) and green seaweed (Ulva lactuca Linnaeus, 1753) in a closed recirculating aquaculture system. Aquaculture, 322-323, 201-209. https://doi.org/10.1016/j.aquaculture.2011.09.030
Kirst, G. O. (1990). Salinity tolerance of eukaryotic marine algae. Annual Review of Plant Physiology and Plant Molecular Biology, 41, 21-53. https://doi.org/10.1146/annurev.pp.41.060190.000321
Kraft, L. G., Kraft, G. T., & Waller, R. F. (2010). Investigations into southern Australian Ulva (Ulvophyceae, Chlorophyta) taxonomy and molecular phylogeny indicate both cosmopolitanism and endemic cryptic species. Journal of Phycology, 46(6), 1257-1277. https://doi.org/10.1111/j.1529-8817.2010.00909.x
Kuhn, D. D., Smith, S. A., Boardman, G. D., Angier, M. W., Marsh, L., & Flick, G. J. (2010). Chronic toxicity of nitrate to Pacific white shrimp, Litopenaeus vannamei: Impacts on survival, growth, antennae length, and pathology. Aquaculture, 309(1-4), 109-114. https://doi.org/10.1016/j.aquaculture.2010.09.014
Legarda, E. C., Crab, L. A., Soares, R., Vinatea, L., Schveitzer, R., & Emerenciano, M. (2021a). Sea lettuce integrated with Pacific white shrimp and mullet cultivation in biofloc impact system performance and the sea lettuce nutritional composition. Aquaculture, 534, 736265. https://doi.org/10.1016/j.aquaculture.2020.736265
Legarda, E. C., Viana, M. T., Zaragoza, O. B. D. R., Skrzynska, A. K., Braga, A., Lorenzo, M. A., & Vieira, F. N. (2021b). Effects on fatty acids profile of Seriola dorsalis muscle tissue fed diets supplemented with different levels of Ulva fasciata from an Integrated Multi-Trophic Aquaculture system. Aquaculture, 535, 736414. https://doi.org/10.1016/j.aquaculture.2021.736414
Lignell, A., & Pedersén, M. (1989). Agar composition as a function of morphology and growth rate. Studies on some morphological strains of Gracilaria secundata and Gracilaria verrucosa (Rhodophyta). Botanica Marina, 32(3), 219-228. https://doi.org/10.1515/botm.1989.32.3.219
Lobban, C. S., & Harrison, P. J. (1994). Seaweed ecology and physiology. Cambridge University Press. https://doi.org/10.1017/CBO9780511626210
Luo, M. B., Liu, F., & Xu, Z. L. (2012). Growth and nutrient uptake capacity of two co-occurring species, Ulva prolifera and Ulva linza. Aquatic Botany, 100, 18-24. https://doi.org/10.1016/j.aquabot.2012.03.006
Martins, M. A., Silva, V. F., Tarapuez, P. R., Hayashi, L., & Vieira, F. N. (2020). Cultivation of the seaweed Ulva Spp. with effluent from a shrimp biofloc rearing system: diferente species and stocking density. Boletim do Instituto de Pesca, 46(3), e602. https://doi.org/ 10.20950/1678-2305.2020.46.3.602
Moustafa, Y. T. A., & Eladel, H. M. (2016). Amino acid profile of Ulva sp. macroalgae collected off the coast of Alexandria, Egypt: a potential food resource. Point Journal of Botany and Microbiology Research, 1(2), 15-22.
Morais, A. P., Santos, I., Carneiro, R. F. S., Routledge, E. A., Hayashi, L., Lorenzo, M. A., & Vieira, F. B. (2023). Integrated multitrophic aquaculture system applied to shrimp, tilapia, and seaweed (Ulva ohnoi) using biofloc technology. Aquaculture, 572, 739492. https://doi.org/10.1016/j.aquaculture.2023.739492
Naldi, M., & Wheeler, P. A. (2002). 15N measurements of ammonium and nitrate uptake by Ulva fenestrata (Chlorophyta) and Gracilaria pacifica (Rhodophyta): Comparison of net nutrient disappearance, release of ammonium and nitrate, and 15N accumulation in algal tissue. Journal of Phycology, 38(1), 135-144. https://doi.org/10.1046/j.1529-8817.2002.01070.x
Notoya, M. (1999). Utilization of Ulva spp. and environmental restoration. Seizandou.
Porra, R. J., Thompson, W. A., & Kreidemann, P. E. (1989). Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: Verification of the concentration of chlorophyll standards by atomic absorption spectrometry. Biochimica et Biophysica Acta, 975(3), 384-394. https://doi.org/10.1016/S0005-2728(89)80347-0
Ritchie, R. J. (2006). Consistent sets of spectrophotometric chlorophyll equations for acetone, methanol and ethanol solvents. Photosynthesis Research, 89, 27-41. https://doi.org/10.1007/s11120-006-9065-9
Robertson-Andersson, D. V. (2003). The cultivation of Ulva lactuca (Chlorophyta) in an integrated aquaculture system, for the production of abalone feed and the bioremediation of aquaculture effluent [MSc Dissertation]. University of Cape Town. http://hdl.handle.net/11427/6175
Rocha, J. S., Santos, D., Martins, M. A., Bauer, C. M., Maraschin, M., Hayashi, L., & Vieira, F. N. (2023). Sea lettuce (Ulva ohnoi) cultivation in biofloc technology: growth performance and characterization of bioactive compounds. Boletim do Instituto de Pesca, 49, e848. https://doi.org/10.20950/1678-2305/bip.2023.49.e848
Rybak, A. S. (2015). Revision of herbarium specimens of freshwater Enteromorpha-like Ulva (Ulvaceae, Chlorophyta) collected from Central Europe during the years 1849–1959. Phytotaxa, 218(1), 1-19. https://doi.org/10.11646/phytotaxa.218.1.1
Santelices, B., Aedo, D., & Hoffmann, A. (2002). Banks of microscopic forms and survival to darkness of propagules and microscopic stages of macroalgae. Revista Chilena de Historia Natural, 75(3), 547-555. https://doi.org/10.4067/S0716-078X2002000300006
Schiavon, M., Moro, I., Pilon-Smits, E. A., Matozzo, V., Malagoli, M., & Dalla Vecchia, F. (2012). Accumulation of selenium in Ulva sp. and effects on morphology, ultrastructure and antioxidant enzymes and metabolites. Aquatic Toxicology, 122-123, 222-231. https://doi.org/10.1016/j.aquatox.2012.06.014
Sheppard, E. J., Hurd, C. L., Britton, D. D., Reed, D. C., & Bach, L. T. (2023). Seaweed biogeochemistry: Global assessment of C:N and C:P ratios and implications for ocean afforestation. Journal of Phycology, 59(5), 879-892. https://doi.org/10.1111/jpy.13381
Silva, P. H., McBride, S., Nys, R., & Paul, N. A. (2008). Integrating filamentous ‘green tide’ algae into tropical pond-based aquaculture. Aquaculture, 284(1-4), 74-80. https://doi.org/10.1016/j.aquaculture.2008.07.035
Simon, C., McHale, M., & Sulpice, R. (2022). Applications of Ulva biomass and strategies to improve its yield and composition: A perspective for Ulva aquaculture. Biology, 11(11), 1593. https://doi.org/10.3390/biology11111593
Steinhagen, S., Johansson, I., Specht, J., Enge, S., Larsson, K., Undeland, I., & Toth, G. B. (2025). Unlocking economic potential of the Ulva crop for low salinity environments: Exploring the effect of salinity gradients on the performance and valuable compounds of Baltic Sea strains. Botanica Marina, 68(1), 65-82. https://doi.org/10.1515/bot-2024-0079
Strickland, J. D. H., & Parsons, T. R. (1972). A practical handbook of seawater analysis. Fisheries Research Board of Canada.
Taylor, R., Fletcher, R. L., & Raven, J. A. (2001). Preliminary studies on the growth of selected ‘green tide’ algae in laboratory culture: Effects of irradiance, temperature, salinity and nutrients on growth rate. Botanica Marina, 44(4), 327-336. https://doi.org/10.1515/BOT.2001.042
United Nations Educational, Scientific and Cultural Organization (UNESCO) (1983). Chemical methods for use in marine environmental monitoring (No. 53). UNESCO.
Zar, J. H. (2010). Biostatistical analysis (5th ed.). Prentice Hall.
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Copyright (c) 2026 Ana Paula Mariane de Morais, Ivanilson de Lima Santos, Carmen Simioni, Leila Hayashi, Marco Antônio de Lorenzo, Walter Quadros Seiffert, Felipe Vieira
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