Inflamação aguda provocada por carragenina em pacu, Piaractus mesopotamicus (Osteichthyes: Characidae), cultivado no Brasil

Autores

  • Maurício Laterça Martins Departamento de Aq-­¼icultura, CCA, Universidade Federal de Santa Catarina (UFSC) - SC, Brasil http://orcid.org/0000-0002-0862-6927
  • Flávio Ruas de Moraes Departamento de Patologia Veterinária, Universidade Estadual Paulista (UNESP) - Jaboticabal, SP, Brasil http://orcid.org/0000-0001-6607-8650
  • Rodrigo Yudi Fujimoto Núcleo de Estudos Costeiros, Universidade Federal do Pará (UFPA), Campus de Bragança - Belém, PA, Brasil
  • Eduardo Makoto Onaka Instituto de Pesca, Centro Avançado de Pesquisa Tecnológica do Agronegócio do Pescado Continental - São José do Rio Preto, SP, Brasil http://orcid.org/0000-0003-2539-0036
  • Fabiana Rizzi Bozzo Departamento de Patologia Veterinária, Universidade Estadual Paulista (UNESP) - Jaboticabal, SP, Brasil
  • Julieta Rodini Engrácia de Moraes Departamento de Patologia Veterinária, Universidade Estadual Paulista (UNESP) - Jaboticabal, SP, Brasil http://orcid.org/0000-0002-8384-8781

Palavras-chave:

Piaractus mesopotamicus, inflamação, carragenina, estresse consecutivo, cortisol, glicemia, hematologia

Resumo

Este trabalho avaliou a resposta inflamatória aguda induzida por injeção de carragenina (500 µg) na bexiga natatória de pacu (Piaractus mesopotamicus) após estresse consecutivo de captura. Os animais foram submetidos a exposição fora da água por 30 segundos, repetindo-se o processo a cada 60 minutos num perí­­odo de seis horas. Seis horas depois das injeções de solução salina (controle) ou carragenina, o componente da resposta inflamatória foi analisado pela contagem total e diferencial de células. O cortisol e a glicemia foram analisados a cada hora. A análise do exsudato inflamatório mostra predominí­¢ncia de trombócitos, seguidos por macrófagos. Significativa redução dos ní­­veis de cortisol observa-se nos animais após o terceiro estí­­mulo estressante, bem como aumento da glicemia após o segundo estí­­mulo. A contagem diferencial de leucócitos e trombócitos circulantes mostra maior porcentagem de células granulocí­­ticas especiais e significativa neutrofilia após o estresse. Os animais que receberam injeção de carragenina apresentaram neutrofilia e linfocitopenia. Na bexiga natatória, a injeção de carragenina provocou congestão, hemorragia intersticial, dissociação dos feixes de colágeno e infiltrado inflamatório.

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2018-10-29

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