Uso de hidróxido de cálcio no controle de vibrionáceas em viveiros de cultivo do camarão Litopenaeus vannamei em Santa Catarina

José Luiz Pedreira Mouriño; Celso Carlos Buglione Neto; Felipe do Nascimento Vieira; Cristina Ramirez; Walter Quadros Seiffert; Maurício laterça Martins; Fabiola Santiago Pedrotti; Rodrigo Schveitzer

Authors

José Luiz Pedreira Mouriño Laboratório de Camaríµes Marinhos (LCM), CCA, Universidade Federal de Santa Catarina http://orcid.org/0000-0002-8619-0882
Celso Carlos Buglione Neto Graduando em Engenharia de Aq-¼icultura, CCA, Universidade Federal de Santa Catarina
Felipe do Nascimento Vieira Mestrando em Aq-¼icultura, Universidade Federal de Santa Catarina
Cristina Ramirez Universidad Del Valle, Calle 13, no 100-00, Cali, Colombia
Walter Quadros Seiffert Laboratório de Camaríµes Marinhos (LCM), CCA, Universidade Federal de Santa Catarina
Maurício laterça Martins Laboratório de diagnóstico e Patologia Aquática, CCA, Universidade Federal de Santa Catarina http://orcid.org/0000-0002-0862-6927
Fabiola Santiago Pedrotti Graduando em Engenharia de Aq-¼icultura, CCA, Universidade Federal de Santa Catarina http://orcid.org/0000-0002-8467-8630
Rodrigo Schveitzer Laboratório de Camaríµes Marinhos (LCM), CCA, Universidade Federal de Santa Catarina http://orcid.org/0000-0002-5660-3930

Keywords:

Vibrio, Litopenaeus vannamei, calcium hydroxide

Abstract

Bacterial diseases, mainly those caused by bacteria of the Vibrio genus, are responsible for significant economic losses in the shrimp production. This study aimed to verify the concentration of calcium hydroxide [Ca(OH)₂] able to inhibit growth of Vibrio harveyi in vitro, and also to test in vivo its use in culture ponds. For the in vitro experiment, bottles were used with BHI culture medium (Brain Heart Infusion) supplemented with Ca(OH)₂ in four different concentrations (0; 0.018 g/L; 0.025 g/L and 0.036 g/L), inoculated with 1.2 x 10¹¹CFU/mL and incubated for 24 h under continuous agitation. The bacterial growth in the treatments was estimated by total counting of colonies in plates by successive dilutions (1/10). For the experiment in culture ponds, calcium hydroxide in the concentration of 0.018 g/L was applied for three consecutive days. Total counting of colonies in Marine Agar and TCBS (Thiosulphate-Citrate-Bile Salts-Sucrose) plates was done before and 48 h after calcium hydroxide application. The Vibrio concentration decreased significantly after the application, from 4.36 Â± 0.1 (log UFC/mL Â± DP) to 3.07 Â± 0.36 (log UFC/mL Â± DP). However, significant decrease in the concentration of total bacteria was not observed. In this way, it was evidenced that Ca(OH)₂ can be used in the control of Vibrio spp. in rearing systems.

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